PCNA Expression In Epithelial Linings Of Odontogenic Cysts

Authors

  • Janti Sudiono Pathology Anatomy Department, Faculty of Dentistry, Trisakti University
  • Rosnah Binti Zain Department of Oral Pathology, Oral Medicine and Periodontology, Faculty of Dentistry, University Malaya

DOI:

https://doi.org/10.22452/adum.vol10no1.1

Keywords:

immunostaining, PCNA, odontogenic cysts

Abstract

Proliferating Cell Nuclear Antigen (PCNA) is one of the several markers of cellular proliferation. Epithelial proliferations play a significant role in the behaviour of odontogenic lesions. The objective of this study was to describe and compare the distribution of PCNA expression within the epithelial linings of odontogenic cysts. A total of 49 cases of odontogenic cysts consisting of 18 radicular cysts, 16 dentigerous cysts, 15 odontogenic keratocysts (OKCs) was studied. All tissues were processed routinely prior to embedding in paraffin. PCNA immunohistochemical staining was performed on 4 !-tm thick deparaffinized sections mounted on sialinized slides using the peroxidase antiperoxidase method. The distributions of PCNA expression in the cysts linings were noted and comparison was made qualitatively and quantitatively. PCNA labelling index was used for the quantitative assessment. The results showed that PCNA staining was distributed in the basal and supra basal cells for radicular cysts, dentigerous cysts, and OKCs. PCNA labelling index was highest in OKC (22.33?4.07). The high PCNA labelling index in OKC is indicative of high proliferative activity thus supporting previous reports of OKC as the most aggressive type of odontogenic cysts.

Downloads

Download data is not yet available.

Downloads

Published

2003-12-31

Issue

Vol. 10 No. 1 (2003)

Section

Original/Research Article

License

  1. The Faculty of Dentistry, Universiti Malaya holds the copyright of the published manuscript including all its components such as supplementary information, graphical contents and raw data without any restrictions.
  2. The author grant the Faculty of Dentistry, University of Malaya assignable and sub-licensable right, unlimited in time and territory, to copy-edit, reproduce, publish, distribute, transmit, make available and store the Article, including abstracts thereof, in all forms of media of expression now known or developed in the future, including pre- and reprints, translations, photographic reproductions and extensions.
  3. After acceptance for publication in Ann Dent UM, the author(s) have the right to use any of its components in whole or in part for academic purposes such as, presentation in scientific conferences and seminars.