Alkaline phosphatase activity assessment of two endodontic materials: a preliminary study

Authors

  • S. Rajan Department of Children's Dentistry & Orthodontics, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.
  • H. Awang Department of Children's Dentistry & Orthodontics, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.
  • S. Devi Department of Medical Microbiology,Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.
  • A.H. Pooi Institute of Mathematical Science
  • H. Hassan Department of Medical Microbiology

DOI:

https://doi.org/10.22452/adum.vol15no1.2

Keywords:

MTA, calcium hydroxide, alkaline phosphatase, in vitro assessment, MG-63 cell line

Abstract

      Objective: An in vitro assessment of MG-63 human osteosarcoma cells' alkaline phosphatase (ALP) activity when in contact with calcium hydroxide powder (CH), paste (CHP) and grey mineral trioxide aggregate (MTA).
      Methods: MG-63 cells were seeded to the three selected materials for durations of 0.25, 0.5, 1, 24, 48 and 72 hours. BCIP-NBT assay was used and ALP activity quantified using ELISA reader at 410 nm.
      Results: The overall analysis for ALP activity indicated significant interaction between test materials and control (maintenance medium). Subsequently, the test materials were paired and analysed for initial (0.25, 0.5, 1 hour) and delayed response (24, 48 and 72 hours). During the initial response, CH exhibited an increased ALP activity compared to MTA. This interaction was not dependant on duration. The delayed response exhibited elevated ALP activity with CHP when
compared to MTA and CH. The interaction of CHP was dependant on duration.
      Conclusion: All three materials exhibited
increased ALP activity.

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Published

2008-06-30

Issue

Vol. 15 No. 1 (2008)

Section

Articles

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